Kidd Lab 

RsaI site(=NCAMFAR656)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     NCAMFARF:     5'-GCT GCT TTG CCT TGT TTG CC-3'               100 ng/reaction
Primer 2.     NCAMFARR:     5'-TGC TGG GGA TT ATC TGT GAG-3'             100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  95^oC (5')
                               95^oC (30"), 60^oC (30"), 72^oC (1')  × 30 cycles
                               72^oC (10')

Product size:
                                    RsaI
                   1145 bp  -------------> 514 bp + 474 bp with a constant band of 157 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies