Kidd Lab 

HhaI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     NCAMRS5F:      5'-GAC TGC TTA CCC TCT GTG T-3'            100 ng/reaction
Primer 2.     NCAMRS5R:      5'-CAA TTC GTC AGT TAT CCC AGG-3'         100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (5')
                              94^oC (1'), 66^oC (30"), 72^oC (1.5')  × 30 cycles

Product size:
                                HhaI
                   1625 bp--------------> 1625 bp and 1087 bp + 538 bp (in heterozygote)
 
 

Kidd Lab Histograms for Allele Frequencies