Kidd Lab 

BsaI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     NCAMBSAIF:     5'-ATG CAA GCT GGA TCA GGT GTC-3'               100 ng/reaction
Primer 2.     NCAMBSAIR:     5'-TTC TTC AAT GGG GTC TTT C-3'                      100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  95^oC (5')
                               95^oC (30"), 60^oC (30"), 72^oC (1')  × 35 cycles
                               72^oC (10')

Product size:
                                    BsaI
                   785 bp  -------------> 474 bp + 311 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies