Kidd Lab 

BamHI site

Template: Genomic DNA 100-200 ng/reaction volume of 20 µl

Primer 1.     NCAMBAMF:     5'-TGG CTA TTC TAA GTA CCT CAC AT-3'         100 ng/reaction
Primer 2.     NCAMINT12:     5'-CAG GCC CAC GAT GGT GAC GA-3'              100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  95^oC (5')
                               95^oC (30"), 55^oC (30"), 72^oC (1')  × 30 cycles
                               72^oC (10')

Product size:
                                  BamHI
                   566 bp  ----------------> 383 bp + 183 bp (in homozygote for site present)
 
 

Kidd Lab Histograms for Allele Frequencies