Kidd Lab 

(CA)_n site (4.4 kb upstream of HOXB6; Clone pH 2-13-2, pBS2)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     PYG1Mod*:     5'-CAG TTC GGG AGT AAA ATT CTT G-3'          Final Concentration 0.16 µM
Primer 2.     PYG8        :     5'-GAA ATT GGA AGC CTG GAC-3'                   Final Concentration 0.16 µM
                  * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 58^oC (30"), 72^oC (1')  × 30 cycles

Product size: Varies from 128 - 154 bp.

Reference:
Deinard, A.S., G. Ruano, and K.K. Kidd, 1992. A dinucleotide repeat polymorphism at the HOX2B locus. Nucleic Acids Research 20:1171.
 
 

Kidd Lab Histograms for Allele Frequencies