Kidd Lab 

TaqI site (Clone pH 2-1-5)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     H2GCK3:       5'-TAT TTT TGT GAT CAA TGG AGA CC-3'         100 ng/reaction
Primer 2.     H2GCK3R:     5'-ACA GAG TCC CTC ACT GCT G-3'                100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 60^oC (30"), 72^oC (1')  × 30 cycles

Product size:
                                 TaqI
                   ~316bp ----------->200 bp + 116 bp (in homozygote site present)

Reference:
Ogura, T., C.M. Castiglione, A.J. Pakstis, and K.K. Kidd, 1991. Two RFLPs at the HOX2G locus. Nucleic Acids Research 19:1716.
 
 

Kidd Lab Histograms for Allele Frequencies