Kidd Lab 

(ACAG)_{1 or 2} Tandem Duplication (located 600 bp downstream of HOXB3 exon 4)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     H2GEX4BK1*:     5'-CTG CAG GCC CAG ACA TCT C-3'           Final Concentration 0.16 µM
Primer 2.     H2GE4D2B:        5'-CTT GAC TTA ACG TGA AAA CAG-3'        Final Concentration 0.16 µM
                  * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 58^oC (30"), 72^oC (1')  × 35 cycles

Product size: Two alleles have been observed: 166 bp (no duplication) or 170 bp (duplication).

Reference:  Discovered in Kidd Lab by resequencing.
 
 

Kidd Lab Histograms for Allele Frequencies