Kidd Lab 

Pentanucleotide insertion/deletion (located approx. 6.7 kb downstream of HOXB1)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     HBXBDELF*:   5'-CCA GCT GCA GTG GGA TAA T-3'          Final Concentration 0.16 µM
Primer 2.     HBXBSNPR:    5'-TTG TAG GAA GTC AGG AGA CC-3'       Final Concentration 0.16 µM
                  * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 58^oC (30"), 72^oC (1')  × 30 cycles

Product size: Two alleles have been observed: 125 bp and 130 bp.

Reference:  Discovered in Kidd Lab by resequencing.
 
 

Kidd Lab Histograms for Allele Frequencies