Kidd Lab 

TaqI (A) site (located >10 kb downstream from exon 8)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     5014MOD:     5'-CCT TCC TGA GTG TCA TCA AC-3'        100 ng/reaction
Primer 2.     TAQACK1:     5'-ACG GCT CCT TGC CCT CTA G-3'         100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (5')
                              94^oC (30"), 60^oC (30"), 72^oC (1')  × 30 cycles

Product size:
                                TaqI
                   236 bp--------------> 124 bp + 112 bp (in homozygote site present)

Reference:
Grandy, D.K., Y. Zhang, and O. Civelli, 1993. PCR detection of the TaqI A RFLP at the DRD2 locus. Hum. Mol. Genet. 2:2197.
 
 

Kidd Lab Histograms for Allele Frequencies