Kidd Lab 

HincII site (located 1.9 kb downstream from exon 8)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     D2EX8F9:     5'-GAG CAA TCT TGT GCT CCA G-3'            100 ng/reaction
Primer 2.     D2Hinc2R:    5'-GGA GAA AGG TGC CTT GGA AG-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (5')
                              94^oC (30"), 60^oC (30"), 72^oC (1')  × 30 cycles

Product size:
                                HincII
                   190 bp--------------> 140 bp + 50 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies