Kidd Lab 

BslI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     RS2TYPF:      5'-CAC CAG CAG CCC TGC CTA TC-3'            100 ng/reaction
Primer 2.     RS2TYPR:      5'-TCT GGA GGC CCC TGT TTC TT-3'         100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (4')
                              95^oC (30"), 56^oC (30"), 72^oC (1.0')  × 30 cycles
                              72^oC (7.0')

Product size:
                                Bsl I
                   291 bp--------------> 291 bp and 197 bp + 94 bp (in heterozygote)

SNP found by resequencing. Located in intron 1 of DRD2 17kb upstream of TaqI "B" site.
 

Kidd Lab Histograms for Allele Frequencies