Kidd Lab 

(CT)_n site (located in intron 1, approixmately 8 kb upstream from exon 2)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.    * CT-D2F:    5'-GGC AGG TCC TGG GAG AAC AC-3'            100 ng/reaction
Primer 2.      CT-D2R:    5'-CAG ATG AAA TAG AAA TGA GGT G-3'        100 ng/reaction
                 * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 1 Unit/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (30"), 60^oC (30"), 70^oC (15")  × 23 cycles

Product size:  116-124 bp
 
 

Kidd Lab Histograms for Allele Frequencies