Kidd Lab 

(CA)_n site (located in intron 2, ~1.25 kb upstream from exon 3)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.    * DRD2CA FOR:        5'-TGT CCT TGG TAG GAT GGG A-3'        100 ng/reaction
Primer 2.      DRD2CA REV1:      5'-GGA GGG CGG TGC GT-3'                    100 ng/reaction
                   * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (5')
                              94^oC (30"), 58^oC (30"), 70^oC (1')  × 30 cycles

Product size:  Varies from 114-120 bp
 
 

Kidd Lab Histograms for Allele Frequencies