Kidd Lab 

Alu deletion site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.    405:    5'-CTG TAT ACT CAG CTA CTA GGG T-3'         50 ng/reaction
Primer 2.    491:    5'-AAA TAG GCT GGA CCG CGG-3'                 50 ng/reaction
Primer 3.    486:    5'-CTC AGG GGT TAT CTA AAG TGG C-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (1'), 60^oC (1.5'), 72^oC (1.5')  × 30 cycles

Product size: 1008 bp and 494 bp for heterozygous (+/-) sample

Protocol from:  Mahadevan MS, Foitzik MA, Surh LC, and Korneluk RG, 1993.  Characterization and Polymerase chian reaction (PCR) detection of an Alu deletion polymorphism in total linkage disequilibrium with myotonic dystrophy. Genomics15: 446-448.
 
 

Kidd Lab Histograms for Allele Frequencies