Kidd Lab 

PstI (B) site (located 700 bp upstream (telomeric) of the HindIII (A) site)

Template: Genomic DNA 100-200 ng/reaction volume of 20 µl

Primer 1.     TV16RPIN1:      5'-CCT TGG GTG GAT ACT TAC C-3'             100 ng/reaction
Primer 2.     H3AR3:            5'-CCA CGT TCT AAT TTC CCC AGT T-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM/reaction

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 54^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                 PstI
                    715 bp--------------> 292 bp + 221 bp (in homozygote site present)
                                                                        There is a constant band of 202 bp.

Reference: Discovered in Kidd Lab by resequencing.
 
 

Kidd Lab Histograms for Allele Frequencies