Kidd Lab

Department of Genetics, Yale University

D4S10 MspI (C) Polymorphism

MspI (C) site (toward telomere end of locus, 13 kb telomeric of MspI (A). This is evidently an Africa specfic polymorphism.)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1. 856MspF: 5'-GAC TAA GCC AGA CTC CAG AC-3' 100 ng/reaction
Primer 2. 856LR4: 5'-TCC TGC CCA CCT CCC TGA AG-3' 100 ng/reaction

dNTPs: Final Concentration 200 µM/reaction

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
95^oC (30"), 61^oC (30"), 72^oC (45") × 30 cycles

Product size:
                                 MspI
                    403 bp-----------------------------> 332 bp + 71 bp (site absent)
                                 MspI
                    403 bp-----------------------------> 226 bp + 106 bp + 71 bp (site present)

Kidd Lab Histograms for Allele Frequencies

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last modified: 11/14/2002