Kidd Lab 

(CA)_n site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.      D12S1344CAF:        5'-[TET]TTC AGG TGG TAC AGC CGT G-3'                 100 ng/reaction
Primer 2.      D12S1344CAR:        5'-CAT CAG AAG GCT TCA TAG GAA TA-3'                100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.0 mM MgCl₂, 50 mM KCl, 15 mM Tris-HCl pH 8.0

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (10')
                              94^oC (30"), 57^oC (30"), 70^oC (1')  × 35 cycles
                              72^oC (10')

Product size:  Varies from 222 to 250 bp
 
 

Kidd Lab Histograms for Allele Frequencies