Kidd Lab 

TaqI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     S94TAQF:     5'-CTG GAA CAC GCT CGG AAC TC-3'            90 ng/reaction
Primer 2.     S94TAQR:     5'-TGA ATT TAT GCC AAG ATG GCT C-3'        90 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 60^oC (30"), 72^oC (30")  × 30 cycles
                              72^oC (7')

Product size:
                                  TaqI
                    475 bp--------------> 340 bp + 117 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies