Kidd Lab 

PstI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     S94PSTF:     5'-TTC CTT GTC CCT AAG TTA TCT-3'         100 ng/reaction
Primer 2.     S94PSTR:     5'-TTC CTT GTC CCT AAG TTA TCT C-3'         100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 57^oC (30"), 72^oC (30")  × 30 cycles
                              72^oC (7')

Product size:
                                  PstI
                    414 bp--------------> 250 bp + 164 bp (in homozygote site present)
 
 

Kidd Lab Histograms for Allele Frequencies