Kidd Lab

Department of Genetics, Yale University

D10S20 RsaI Polymorphism

RsaI site

Template: Genomic DNA 100-200 ng/reaction volume of 20 µl

Primer 1. OS3TESTB: 5'-TCA TTC CAT TTA CAT CTA CTT GGG-3' 100 ng/Reaction
Primer 2. 1020BANR: 5'-GAT GCA ATC TAG AAA CAG TCG-3' 100 ng/Reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
(Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
95^oC (30"), 57^oC (30"), 72^oC (1') × 30 cycles

Product size:
                                     RsaI
                    1506 bp----------------> 619 bp + 739 bp (in homozygote site present)
                                                    with constant 85 bp and 63 bp bands

Kidd Lab Histograms for Allele Frequencies

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last modified: 07/21/2002