Kidd Lab 

HindIII site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     D10S20HNF:     5'-TGC AAG AAT TGA GAG ACT AAT C-3'        100 ng/reaction
Primer 2.     D10S20HNR:     5'-ATA CAG TAG CCA CAT CAT AGA-3'           100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 58^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                  HindIII
                    391 bp----------------> 210 bp + 181 bp (in homozygote for the HindIII site)
 
 

Kidd Lab Histograms for Allele Frequencies