Kidd Lab 

BglI site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     COMTBGL1F:    5'-TGC GGA AGG GGA CAG TGC TAC-3'                   100 ng/reaction
Primer 2.     COMTBGL1R:    5'-CCG GAG CCG CAG AAG GTC A-3'                       100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 60^oC (30"), 72^oC (30")  × 30 cycles

Product Size:
                                Bgl I
                   278 bp -------------> 278 bp and 196 bp + 82 bp in heterozygote
 
 
 

Reference:
Chen,C-H, Y-R Lee, M-Y Liu, F-C Wei, F-J Koong, H-G Hwu, and K-J Hsiao, 1996.  Identification of a BglI polymorphism of Catechol-O-Methyltransferase (COMT) gene, and association study with schizophrenia.  Am. J. Med. Genet. (Neuropsychiatric Genetics) 67: 556-559.
[Note: the reverse primer is not correct in the article; in this reference, the two primers given are accidentally the same.  The primers on this listing work in this protocol. The fragment sizes given here are those obtained with these primers.]
 
 

Kidd Lab Histograms for Allele Frequencies