Kidd Lab 

Pentanucleotide Repeat site (located in intron 5)

Template: Genomic DNA 100-200 ng/reaction volume of 20 µl

Primer 1.       CD4 A:     5'-TTG GAG TCG CAA GCT GAA CTA GC-3'              Final Concentration 0.16  µM
Primer 2.     * CD4 B:     5'-GCC TGA GTG ACA GAG TGA GAA CC-3'            Final Concentration 0.16  µM
                  * kinased ³²P

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 55^oC (1'), 68^oC (1')  × 30 cycles

Product size: Varies from 85-130 bp
 
 

Kidd Lab Histograms for Allele Frequencies