Kidd Lab 

Deletion site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     SP4.760:    5'-CTT CAT TAC ACC TGC AGC TCT-3'            100 ng/reaction
Primer 2.     PM6.942:    5'-CAC AGC CCT GTG CCT CTT CTT C-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 µl
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC, 5'
                              95^oC (1'), 60^oC (1.5"), 72^oC (1.5')  × 30 cycles

Product size: 200 bp and 180 bp for heterozygous (+/-) sample.
 
 

Kidd Lab Histograms for Allele Frequencies