Kidd Lab 

BsaBI site(located about 10kb upstreamof the indel)

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     1604CCR57:    5'-CAG CCA CAG CAG GAT TTT ATT G-3'            100 ng/reaction
Primer 2.     CCR5SNPUR:    5'-TCA TGC ACT GTC TTC CAG AGG T-3'        100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction volume of 25 µl
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC( 5')
                              95^oC (30"), 62^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                BsaB I
                   627 bp--------------> 349 bp + 278 bp (in homozygote site present)

Kidd Lab Histograms for Allele Frequencies