Kidd Lab 

PvuII site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     APOBINT4D:    5'-AAT CTT TGT ATG ACT CTG TTT GC-3'        100 ng/reaction
Primer 2.     APOBEX5U:    5'-CAG CTT GAG CTC ATA CCT GTC-3'             100 ng/reaction

dNTPs: Final Concentration 200 µM/reaction

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 95^oC (5')
                              95^oC (30"), 59^oC (30"), 72^oC (30")  × 30 cycles

Product size:
                                 PvuII
                   713 bp ---------------> 713 bp and 538 bp + 175 bp in heterozygote
 
 

Kidd Lab Histograms for Allele Frequencies