Kidd Lab 

HaeIII(A) site (located in intron 6)

Template: Genomic DNA 100-200 ng/reaction volume of 20 µl

Primer 1.     ALDHIN6AF:     5'-AAA TAT TGC TCT AGG CCA GGC-3'               100 ng/reaction
Primer 2.     ALDHIN6AR:     5'-TGG GAA TTC TAA ATG GGA CGG-3'              100 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 1.5 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4, 1 M Betaine

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile: 94^oC (5')
                              94^oC (30"), 57^oC (30"), 72^oC (30")  × 35 cycles
                             72^oC (10')

Product size:
                                       HaeIII
                         401 bp ---------------->226 bp + 116 bp + 44 bp + 15 bp (in homozygote for site present)
                                       HaeIII
                         401 bp ---------------->342 bp + 44 bp + 15 bp (in homozygote for site absent)
 
 

Kidd Lab Histograms for Allele Frequencies