Kidd Lab 

Alu Insertion site

Template: Genomic DNA 100-200 ng/reaction volume of 25 µl

Primer 1.     SC1407:   5'-CTG GAG ACC ACT CCC ATC CTT TCT-3'          75 ng/reaction
Primer 2.     BR2352:   5'-GAT GTG GCC ATC ACA TTC GTC AGA T-3'      75 ng/reaction

dNTPs: Final Concentration 200 µM each

Buffer: Final Concentration 2.25 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl pH 8.4

Taq DNA Polymerase: 0.5 Units/reaction
                                 (Perkin-Elmer Amplitaq DNA polymerase 5 U/µl, Cat. No. N808-0145)

PCR Cycling Profile:  94^oC (5')
                               94^oC (30"), 58^oC (30"), 72^oC (30")  × 30 cycles

Product size: 490 bp + 190 bp  (+/- individual)

Reference:
Tiret et al., 1992.  Evidence from combined segregation and linkage analysis that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels.  Amer. J. hum. Genet. 51: 197-205.
 
 

Kidd Lab Histograms for Allele Frequencies