Leonard M. Milstone, M.D.

Professor of Dermatology

Research Interests

My research focuses on the biology of normal and abnormal keratinocytes. We study disorders of keratinization in patients who have ichthyosis and, in the laboratory, in animal models and cultured epidermal keratinocytes. Active projects in the lab involve 1) molecular targeting of genes to correct specific gene defects; 2) dermatoremediation of systemic disease, for example by harnessing epidermal desquamation to treat iron overload.

Education:	1966, B.S. Yale University 1970, M.D. Yale University
Training:	Residency: University of Oregon, Yale University Fellowship: NIH

Selected Publications

• Milstone LM, Hough-Monroe L, Kugelman LC, Bender JR, Haggerty JG. Epican, a heparin chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion. J Cell Sci 107: 3183-3190, 1994.
• Zhou J, Haggerty JG, Milstone LM. Growth and differentiation regulate CD44 expression on human keratinocytes. In Vitro Cell Develop Biol 35:228-235, 1999.
• Leachman SA, Insogna KL, Katz L, Ellison A, Milstone LM. Bone densities in patients receiving isotretinoin for cystic acne. Arch Dermatol 135:961-966, 1999.
• Gruen AB, Zhou J, Morton KA, Milstone LM. Photodegraded nifedipine stimulates uptake and retention of iron in human epidermal keratinocytes. J Invest Dermatol, 116:774-777, 2001.
• Cserhalmi-Friedman PB, Milstone LM, Christiano AM. Diagnosis of autosomal recessive lamellar ichthyosis with mutations in the TGM1 gene. Brit J Dermatol 144:726-730, 2001.
• Connors JB, Rahil AK, Smith FJD, McLean WHI, Milstone LM. Delayed-onset pachyonychia congenita associated with a novel mutation in the central 2B domain of keratin 16. Brit J Dermatol 144:1058-1062, 2001.
• Book Chapter: Milstone LM. Keratinocyte biology: Chair's summary, In Dermatology at the Millennium; eds. Dyall-Smith D and Marks R. Parthenon Publ Grp Ltd (Carnforth, UK) 1998.
• Milstone LM and Leachman SA. Do retinoids cause osteoporosis. in Proceedings of the Fourth International Symposium on Nutritional Aspects of Osteoporosis. Eds. Burckhardt P, Dawson-Hughes B, Heaney RP, Academic Press, pp. 285-293, 2001
• Milstone, LM. Epidermal Desquamation. J Dermatol Sci, 36:131-140, 2004
• Bruegel-Sanchez VL, Zhou J, LaCivita D, Milstone LM. Long-term murine keratinocyte cultures become tetraploid, yet maintain ability to stratify. J Invest Dermatol, 123:403-404, 2004.
• Leachman SA, Kaspar RL, Fleckman P, Florell SR, Smith FJD, McLean WHI, Lunny DP,
  Milstone LM, van Steensel MAM, Munro CS, O’Toole EA, Celebi JT, Kansky A, Lane EB.
  Clinical and pathological features of pachyonychia congenita. J Invest Dermatol Symp Proc 10:3-17, 2005.
• Milstone LM, Fleckman P, Leachman SA, Leigh I, Paller AS, van Steensel MAV. Treatment of pachyonychia congenita. J Invest Dermatol Symp Proc 10:18-20, 2005.
• Lewin AS, Glazer PM, Milstone LM. Gene therapy for autosomal dominant disorders of
  keratin. J Invest Dermatol Symp Proc 10:47-61, 2005.
• Adams BD, Lazova R, Andrews NC, Milstone LM. Iron in skin of mice with three etiologies of systemic iron overload. J Invest Dermatol, 125:1200-1205, 2005.
• Milstone LM, Adams BD, Zhou J, Bruegel-Sanchez V, Shofner J. Stratum-specific
  expression of human transferrin receptor increases iron in mouse epidermis. J Invest Dermatol 126:648-652, 2006.
• Demerjian M, Crumrine D, Milstone LM, Williams ML, Elias PM. Barrier dysfunction and
  pathogenesis of neutral lipid storage disease with ichthyosis (Chanarin-Dorfman syndrome). J Invest Dermatol, in press

Chapters

• Milstone LM. Keratinocyte biology: Chair's summary, In Dermatology at the Millennium; eds. Dyall-Smith D and Marks R. Parthenon Publ Grp Ltd (Carnforth, UK) 1998.

Contact

Campus Address
Yale School of Medicine
333 Cedar Street
New Haven, CT 06520-8059

E-mail
leonard.milstone@yale.edu

Office Phone
(203) 785-3646