Yale School of Medicine

Dermatology

Dermatology, Yale School of Medicine

Dermatology
PO Box 208030
New Haven, CT 06520-8030

Carole L. Berger, Ph.D.

Carole L. Berger, Ph.D.

Research Scientist, Department of Dermatology

Transimmunization Therapy

Cancer Treatment using Photopheresis graduates to a whole new level.

Research Interests

Immune response in patients with cutaneous T cell Lymphoma, class I peptide profile on neoplastic T cells, heat shock proteins as peptide chaperones, and murine model of tumor vaccination.

Clinical Service

Immunophenotyping of peripheral blood of cutaneous T cell lymphoma patients for diagnostic and prognostic assessment.

Education:
 M.S. Columbia University, 1974
Ed.D. Columbia University, 1976
M. Phil, Columbia University, 1983
Ph.D., Columbia University, 1984

Selected Publications

  • Hanlon D, Berger C, Edelson RL. Photoactivated 8-Methoxypsoralen treatment causes a peptide-dependent increase in antigen display by transformed lymphocytes. Int J Cancer 78: 70-75, 1998.
  • Berger CL, Warburton D, Raafat J. LoGerfo P. and Edelson RL: Cutaneous T-Cell Lymphoma: Neoplasm of T-Cells with Helper Activity. Blood, 53:642, 1979.
  • Edelson RL, Berger CL, Raafat J. and Warburton D: Karyotype Studies of Cutaneous T Cell Lymphoma: Evidence for Clonal Origin. J Invest Dermatol. 73:548, 1979.
  • Kung PC, Berger CL, Goldstein G. LoGerfo P. and Edelson RL: Cutaneous T-Cell Lymphoma: Characterization by Monoclonal Antibodies. Blood57:261, 1981.
  • Berger CL, Morrison SL, Chu A, Patterson JAK, Estabrook A, Takezaki S. Sharon J. Warburton D, Edelson RL: Early Diagnosis of CTCL by Use of Monoclonal Antibodies reactive with Tumor-Associated Antigens. J Clin Invest 70:1205, 1982.
  • Takezaki S. Morrison SL, Berger CL, Chu AC, and Edelson RL: Biochemical characterization of a Differentiation Antigen Shared by Human Epidermal Langerhans Cells and Cortical Thymocytes. J Clin Immunol (2): 128-134S, 1982.
  • Chu A, Goldstein G. Patterson JAK, Berger CL, Takezaki S. and Edelson RL: Thymopoietin-Like Substance in Human Skin. Lancet 2: 766, 1982.
  • Chu A, Patterson JAK, Berger CL, Edelson RL: TCell subpopulations of Cutaneous T-cell Lymphoma (CTCL). J Invest Derm 78:334, 1982.
  • Estabrook A, Berger CL, LoGerfo P. Hardy M, Edelson RL: Antigen Modulation of T Lymphocytes by Monoclonal Antibodies. TransplantProc. 15:651, 1983.
  • Kung PC, Berger CL, Estabrook A, Edelson RL: Monoclonal Antibodies for Clinical Investigation of Human T Lymphocytes. Int J. Dermatol 22:67, 1983.
  • Gasparro FP, Berger CL, and Edelson RL: Effect of Monochromatic W-A light and 8-Methoxypsoralen on Human Lymphocytes Response to Mitogen. Photodermatol 1: 10, 1984.
  • Griffiths-Chu S. Patterson JAK, Berger CL, Edelson RL and CHU AC: Characterization of Immature T-cell Subpopulations in Neonatal Blood. Blood 64: 296, 1984.
  • Murphy RF, Bisaccia E, Cantor RC, Berger CL, Edelson RL: Internalization and acidification of insulin by activated human lymphocytes. Cell Physiol 121:351, 1984.
  • Chu A, Patterson JAK, Berger CL, Vonderheid E, and Edelson RL: In Situ Study of T-cell Subpopulations in Cutaneous T-cell Lymphoma. Cancer 54: 2414, 1984.
  • Berger CL, Cantor C, Welsh J. Dervan P. Gasparro FP, and Edelson RL: Inhibition of Iymphocyte proliferation by water soluble psoralen derivatives. Ann NY Acad Sci 446:80-90, 1985.
  • Yemul SS, Berger CL, Estabrook A, Edelson RL and Bayley H: The Delivery of Phototoxic Drugs to Selected Cells, Annals NY Acad Sci 446:403 -414, 1985.
  • Berger C, Friedman-Kien A, DiFranco M, Rehle T. Ostreicher R. Knobler R. Jaubenstein L, Edelson RL: Tumor-Associated Antigen Present on Lymphocytes from Cutaneous T-cell Lymphoma Patients is also found on Lymphocytes from Acquired Immune Deficiency Syndrome Patients. J Invest Dermatol 87:280-283, 1986.
  • Yemul S. Berger C, Estabrook A, Suarez S. Edelson RL and Bayley H: Selective killing of T-lymphocytes by Phototoxic Liposomes. Proc Natl Acad Sci, 84:246-250, 1987.
  • Edelson RL, Berger CL, Gasparro FP, Jegasothy B. Heald P. Wintroub B. Vonderheid E, Knobler R. Wolff K, Plewig G. McKiernan G. Christensen I, Oster M. Honigsmann H. Wilford H. Kokoschka E, Rehle T. Perez M, Stingl G. Laroche L: Treatment of Leukemic Cutaneous T Cell Lymphoma with Extracorporeal Photoactivated 8-Methoxypsoralen. N Engl J Med 316:297-303, 1987.
  • Shapiro P. Warburton D, Berger C, Edelson R: Clonal Chromosomal Abnormalities in Cutaneous T Cell Lymphoma. Cancer Genet Cvtogenet 28:267-276, 1987.
  • Benn P. Soper L, Eisenberg A, Silver RT, Coleman M, Caccapaglia B. Bennett L, Baird M, Silverstein M. Berger C, Bernhardt B: Utility of molecular genetic analysis of BCR rearrangement in the diagnosis of chronic myeloid leukemia (CML). Cancer Genet Cytogenet29:1-7, 1987.
  • Berger CL, Eisenberg A, Soper L, Chow J. Simone J. Gapas Y. Cacciapaglia B. Bennett L, Edelson RL, Warburton D, and Benn P: Clonal Rearrangements of the T Beta Gene and Dual Genotypes in CTCL. J Invest Dermatol 90: 73, 1988.
  • Pepino P. Berger CL, Fuzesi L, Panza A, Pierson RN, Gutierrez C, Marboe CC, Smith CR, Reemtsma K. Rose EA: Primate cardiac allo and xeno transplantation: modulation of the immune response with photochemotherapy. Eur Surg Res 21: 105, 1989.
  • Perez M. Edelson R. Laroche L, Berger CL: Specific suppression of anti-allograft immunity by vaccination with syngeneic Photoinactivated splenocytes. J Invest Dermatol 92:669-676, 1989.
  • Berger CL, Perez M, LaRoche L, Edelson RL: Inhibition of murine autoimmune disease by reinfusion of syngeneic lymphocytes inactivated with psoralen and ultraviolet A light. J Invest Dermatol 94: 52-57, 1990.
  • Yemul S. Berger CL, Goldstein G. Estabrook A, Katz M, Edelson RL, Bayley H: Use of non-modulating antibody targeted phototoxic liposomes as a selective drug delivery system. Cancer Immunol Immunotherapy 30:317, 1990.
  • Berger CL: Experimental murine and primate models for dissection of the immunosuppressive potential of photochemotherapy in autoimmune disease and transplantation. Yale J Biol Med 62:611 1989.
  • Bisaccia E, Berger C Klainer A. A pilot study of extracorporeal photopheresis in AIDS-related complex.Ann IntMedl 13:270,'90.
  • Berger CL, Edelson RL, Edwards N. Sanchez J. Coppey L, He X, Marboe C, Rose E: Autoregulation of the immune response in autoimmune disease and cardiac transplantation by photoinactivated autologous lymphocytes. Ann NY Acad Sci 636:266, 1991.
  • LaRoche L, Edelson RL, Perez M, Berger CL: Antigen-specific tolerance induced by autoimmunization with photoinactivated syngeneiceffectorcells. AnnNYAcadSci636:113,1991.
  • Rose E, Pepino P. Barr M, Smith C, Ho E, Reed E, Berger C, Reemtsma K: Relation of HLA-antibodies and graft atherosclerosis in human cardiac allograft recipients. J Heart Lung Transplant 11:120, 1992.
  • Heald P. Rook A, Perez M, Wintroub B. Knobler R. Jegasothy B. Gasparro F. Berger C, Edelson R: Treatment of erythrodermic cutaneous T-cell lymphoma with extracorporeal photochemotherapy. J Am Acad Dermatol 27:427-433, 1992.
  • Rose E, Barr M, Xu H. Pepino P. Watkins J. Ratner A, Murphy M, McGovern M, Marboe C, Berger C: Photochemotherapy in human heart transplant recipients at high risk for fatal rejection. J Heart Lung Transplant 11 :746-450, 1992.
  • Bisaccia E, Berger C, DiSpaltro FX, Klainer AS: Extracorporeal photopheresis in the treatment of AIDS-related complex. J AIDS 6:386-392, 1993.
  • Xu H. Kwiatkowski P. Chen J. Kaplon R. Edwards N. Dong X, Berger C, Michler E: Abrogation of baboon natural xenoantibody to pig splenocytes by DLpenicillamine. Transplant 58:1299, 1994.
  • Longley J. Tyrrell L, Lu S-Z, Farrell J. Ding T. Yan S. Sallee D, Heald P. Berger C, Tigelaar R. Edelson R: Malignant and normal T cells show random use of T-cell receptor ol chain variable regions in patients with cutaneous T-cell Iymphoma. J Invest Dermatol 105:62,1995.
  • Gonzalez J. Berger C, Cottrill C, Geller A, Schwartz J. Palagio M, Klainer A, Bisaccia E: Cytolytic response to HIV in patients with HIV disease treated with extracorporeal photochemotherapy: Preliminary studies. Photobiol Photochem 63:558, 1996.
  • Berger CL, Wang N. Christensen I, Longley J. Heald P. Edelson RL: The immune response to class I associated tumor-specific cutaneous T cell lymphoma antigens. J Invest Dermatol 107:392-7, 1996.
  • Hanlon D, Berger C, Edelson RL. 8-MOP-Driven Increases in MHC Class I Associated Cell Surface Antigens. Submitted J Invest Dermatol, 1997.
  • Berger CL, Dong Z, Hanlon D, Bisaccia E, Edelson RL: A lymphocyte cell surface heat shock protein homologous to the endoplasmic reticulum chaperone, immunoglobulin heavy chain binding protein BIP. Int J Cancer 71:1077-1085, 1997.
  • Berger CL, Longley BJ, Imaeda S, Christensen I, Heald P, Edelson RL: Tumor-specific peptides in cutaneous T-cell lymphoma: Association with class I major histocompatibility complex and possible derivation from the clonotypic T-cell receptor. Int J Cancer 76:304-311, 1998.
  • Berger CL, Xu A-L, Hanlon D, Lee C, Schechner J, Glusac E, Christensen I, Snyder E, Holloway V, Tigelaar R, Edelson RL. Large-scale induction of human tumor-loaded dendritic cells. Int J Cancer 91:4438-447, 2001.
  • Berger CL, Hanlon D, Kanada D, Dhodapkar M, Lombillo V, Wang N, Christensen I, Howe G, Crouch J, El-Fishawy P, Edelson R. The growth of cutaneous T-cell lymphoma is stimulated by immature dendritic cells. Blood 99:2929-2938,2002.
  • Berger CL, Tigelaar R, Cohen J, Mariwalla K, Trinh J, Wang N, Edelson RL. Cutaneous T cell lymphoma, malignant proliferation of T-regulatory cells. Blood 105:1640-1647, 2005.
  • Salskov-Iversen M, Berger CL, Edelson, RL. Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading. J Immune Based Therapies and Vaccines 3:http://www.figtherapies.com/content/3/1/4, 2005.
  • Wong HK, Wilson AJ, Gibson HM, Hafner MS, Hedgcock C, Berger CL, Edelson RL, Lim HW. Increased expression of CTLA-4 in PBMC and malignant T-cells from patients with mycosis fungoides (CTCL). J Invest Dermatol 126:212-219, 2006.
  • Nebozhyn M, Loboda A, Kari L, Rook AH, Vonderheid EC, Lessin S, Berger C, Edelson R, Nichols C, Yousef M, Gudipati L, Shang M, Showe Mk, Showe LC. Quantitative PCR on 5 genes reliably identifies CTCL patients with 5-99% circulating tumor cells with 90% accuracy. Blood (in press, 2006).
  • Girardi M, Berger CL, Wilson, LD, Christensen IR, Thompson KR, Glusac EJ, Edelson RL. Transimmunization for cutaneous T cell lymphoma: a Phase I study. Leuk Lymph (in press, 2006).

Review Articles

  • Berger CL, Longley J, Hanlon D, Girardi M, Edelson R. The clonotypic T cell receptor is a source of tumor-associated antigens in cutaneous T cell lymphoma. Ann NY Acad Sci 941:106-123, 2001.
  • Girardi M, Berger C, Hanlon D, Edelson RL. Efficient tumor antigen loading of dendritic antigen presenting cells by Transimmunization. Tech Cancer Res 1:65-69, 2002.
  • Berger CL Hanlon D, Kanada D, Girardi M, Edelson RL. Transimmunization, a novel approach for tumor immunotherapy. Trans Apher Sci 26:205-216, 2002.
  • Berger CL, Edelson RL. Current concepts of the immunobiology and immunotherapy of cutaneous T cell lymphoma: Insights gained through cross-talk between the clinic and the bench. Leuk Lymph 44:1697-1703, 2003.
  • Berger CL, Edelson R. The life cycle of cutaneous T cell lymphoma reveals opportunities for targeted drug therapy. Curr Caner Drug Targ 4:609-619, 2004.
  • Berger CL, Shofner J, Vasquez JG, Mariwalla K, Edelson RL. Cancer Immunotherapy. Cancer Therapy 3: (in press, 2006).

Book Chapter

  • Edelson RL and Berger C. Extracorporeal photochemotherapy. In: Malignant Tumors of the Skin. Chu AC and Edelson RL, eds. Edward Arnold Publishers, London , pp. 396-408, 1999.
  • Berger CL, Mariwalla K, Girardi M, Edelson RL. Advances in understanding the immunobiology and immunotherapy of cutaneous T-cell lymphoma. In Advances in Dermatology, Vol 20. W. D. James and S. Hwang (eds): Mosby Inc., Elsevier, New York, USA pp 217-235, 2004.

Contact

Mailing Address
Yale School of Medicine
333 Cedar Street
New Haven, CT 06520-8059

Office Address

E-mail
carole.berger@yale.edu

Office Phone
(203) 737-4024