Hebert Lab: Cl- transporters

Hebert Laboratory

Department of Cellular and Molecular Physiology

Yale School of Medicine

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Sodium/Potassium coupled chloride transporters

Image: Colocalization of EGFP-tagged Thiazide-sensitive Na+-Cl- co-transporter with FM 4-64 at Xenopus oocyte plasma membrane.

Electroneutral cation-coupled chloride transporters are crucial to chloride absorptive and secretory epithelia, cell volume regulation and the setting of intracellular chloride in excitability in neurons. We cloned some of the members of this family and study how they are regulated by ions, diuretics and cytosolic second messengers.

Image: Colocalization of EGFP-tagged Thiazide-sensitive Na+-Cl-
co-transporter with FM 4-64 at Xenopus oocyte plasma membrane.

Representative publications:

Gamba, G., Miyanoshita, A., Lombardi, M., Lee, W.-S., Hediger, M.A., Lytton, J., and Hebert, S.C.: Molecular cloning, primary structure and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney. J. Biol. Chem., 1994; 269:17713-17722.

Gamba, G., Saltzberg, S.N., Lombardi, M., Miyanoshita, A., Lytton, J., Hediger, M.A., Brenner, B.M. and Hebert, S.C.: Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter. Proc. Natl. Acad. Sci., U.S.A., 1993; 90:2749-2753.

Hebert, S.C., Mount, D.B., and Gamba, G.; Molecular physiology of cation-coupled Cl- cotransport: The SLC12 Family. European J. Physiol. (Pflugers Arch.). SLC Review Series, 2003: In Press.

Wilson, F.H., Kahle, K.T., Sabath, E., Lalioti, M., Rapson, A.K., Hoover, R.S., Hebert, S.C., Gamba, G., and Lifton, R.P.: Molecular pathogenesis of inherited hypertension with hyperkalemia: the Na-Cl co-transporter is inhibited by wild-type but not mutant WNK4. Proc. Natl. Acad. Sci., U.S.A., 2003; 100:680-684.

Meade, P., Hoover, R., Plata, C., V·zquez, N., Bobadilla, N.A., Gamba, G.and Hebert, S.C.: Regulation of the renal-specific mouse Na-K-2Cl cotransporter surface expression by the C-terminal spliced isoform. Am. J. Physiol. - Renal Physiol., 2003; 284: F1145-F1154.

Hoover, R.S., Poch, E., Monroy, A., Vazquez, N., Nishio, T., Gamba, G., and Hebert, S.C.: N-Glycosylation at two sites critically alters thiazide binding and activity of the rat thiazide-sensitive Na+:Cl- cotransporter. J. Am. Soc. Nephrology. 2003;14(2): 271-282.

Plata, C., Meade, P., V·zquez, N., Hebert, S.C. and Gamba, G.: Functional properties of the apical Na-K-2Cl cotransporter isoforms. 2002; J. Biol. Chem. 277:11004-11012.

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Last modified: August 22, 2003