Inside Dyes

Each pixel in the recording receives light from a small portion of a neurons which has been stained by microinjection of the dye into the cell body. After waiting for the dye to spread into the processes, the dye can be used to monitor changes in membrane potential in dendrites and axons.

References

Antic S., Wuskell, J. P., Loew, L., and D. Zecevic (2000). Functional profile of a neuron: temporal and spatial dynamics of electrical activity in situ. J. Physiology (London) Aug 15;527 Pt 1:55-69

Antic, S., Major, G., and Zecevic, D (1999). Fast optical recordings of membrane potential changes from dendrites of pyramidal neurons. J. Neurophysiology, 82: 1615-1621.

Antic, S., Major G., Chen, W., Wuskel, J., Loew L., and Zecevic, D (1997). Fast voltagesensitive dye recording of membrane potential changes at multiple sites on an individual nerve cell in the rat cortical slice. Biological Bulletin, 193: 261.

Zecevic, D (1996). Multiple spike initiation zones in single neurons revealed by voltage-sensitive dyes. Nature, 381, 322-325.

Antic S. and D. Zecevic (1995). Optical signals from neurons with internally applied voltage-sensitive dyes. J. Neuroscience, 15, 1392-1405.

Kogan, A., W. N. Ross, D. Zecevic, and N. Lasser-Ross (1995). Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes. Brain Research, 700, 235-239.

Last Updated Sep-01 01:57 PM ean