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Alfred L.M. Bothwell

 

Immunology; Molecular Cell Biology, Genetics & Development

Professor of Immunobiology and Interdepartmental Program in Vascular Biology & Transplantation

Education

A.B. Washington University 1971
Ph.D. Yale University 1975

Research Interests

Studies of human T cell recognition of human and porcine endothelial cells will focus on both direct and indirect recognition of MHC, constitutive and inducible adhesion molecules and costimulatory pathways. Analysis of in vitro function will be complemented by studies of in vivo model systems using synthetic microvessels, and vessel and skin grafts into Hu/SCID mice to study lymphocyte recruitment, vascular remodeling, and to evaluate allo and xenograft interactions. One major goal of this system is to evaluate the potential of porcine cells as a source of donor cells for xenografts. The synthetic microvessel system is also being used to revascularize islets in hopes of treating diabetes. Separate experiments will address recognition, activation, and signaling in murine lymphocytes. Ly-6 antigens are characteristically tethered to the membrane via GPI anchors and as such would be characteristically located in lipid rafts. The role of Ly-6 antigens in regulating the protein components of lipid rafts is under investigation. Studies of Pax5 knockout mice that accumulate proB progenitor populations will be used to evaluate the role of Ly-6 antigens on hematopoietic differentiation. The role of regulatory T cells in prevention of EAE is also under investigation.

Recent Publications

• Pflugh, D.L., Maher, S.E., and Bothwell, A.L.M. (2002). Ly-6 superfamily members Ly-6A/E, Ly-6C and Ly-6I recognize two potential ligands expressed by B lymphocytes. J. Immunol. 169:5130-5136.
• Zheng, L., Ben, L.-H., Pober, J.S., and Bothwell, A.L.M. (2002). Porcine endothelial cells, unlike human endothelial cells, can be killed by human CTL via Fas ligand and cannot be protected by Bcl-2. J. Immunol. 169:6850-6855.

Contact

E-mail
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